spcas9 protein (Addgene inc)
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spcas9 protein
Spcas9 Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+28b+cas9+his/pET-28b-Cas9-His+(Plasmid+%2347327)/bio_rxiv__64898__2026__03__18__712093-371-0-6
Average 94 stars, based on 61 article reviews
Spcas9 Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+28b+cas9+his/pET-28b-Cas9-His+(Plasmid+%2347327)/bio_rxiv__64898__2026__03__18__712093-371-0-6
Average 94 stars, based on 61 article reviews
spcas9 protein - by Bioz Stars,
2026-09
94/100 stars
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Conjugation Assay:Article Title: A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo. Article Snippet: .. Generation and conjugation of cell-penetrating PS-Cas9 The recombinant Cas9 protein was produced in Escherichia coli. Recombinant:Article Title: A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo. Article Snippet: .. Generation and conjugation of cell-penetrating PS-Cas9 The recombinant Cas9 protein was produced in Escherichia coli. Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. All plasmids and primers used in the cloning experiments and oligonucleotides are summarized in Additional file 1: Table S2 and S3, respectively. Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. All plasmids and primers used in the cloning experiments and oligonucleotides are summarized in Additional file 1: Table S2 and S3, respectively. Article Title: An optimized SpCas9 high-fidelity variant for direct protein delivery Article Snippet: A derivative of pX330 (Addgene #42230) where the sgRNA cassette has been removed by NdeI digestion was used to express SpCas9 in mammalian cells (pX-Cas9). pX-Cas9 plasmids encoding the different mutants were obtained by site-directed mutagenesis of the WT pXCas9 plasmid using the ODNs reported in Table S2. sgRNAs were expressed from a pUC19 plasmid containing a U6-driven sgRNA expression cassette. .. Desired spacer sequences were cloned as annealed ODNs (Table S1) into a double BbsI site immediately upstream of the sgRNA scaffold according to previously published cloning strategies.35 To express SpCas9 in bacterial cells for recombinant protein production, a modified version of the Produced:Article Title: A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo. Article Snippet: .. Generation and conjugation of cell-penetrating PS-Cas9 The recombinant Cas9 protein was produced in Escherichia coli. Plasmid Preparation:Article Title: A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo. Article Snippet: .. Generation and conjugation of cell-penetrating PS-Cas9 The recombinant Cas9 protein was produced in Escherichia coli. Article Title: Simplified Protocol for the Purification of Native Cas Nucleases for DNA-Free Genome Editing Article Snippet: .. 1. Activity Assay:Article Title: A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo. Article Snippet: .. Generation and conjugation of cell-penetrating PS-Cas9 The recombinant Cas9 protein was produced in Escherichia coli. Mutagenesis:Article Title: A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo. Article Snippet: .. Generation and conjugation of cell-penetrating PS-Cas9 The recombinant Cas9 protein was produced in Escherichia coli. Cloning:Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. All plasmids and primers used in the cloning experiments and oligonucleotides are summarized in Additional file 1: Table S2 and S3, respectively. Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. All plasmids and primers used in the cloning experiments and oligonucleotides are summarized in Additional file 1: Table S2 and S3, respectively. Article Title: An optimized SpCas9 high-fidelity variant for direct protein delivery Article Snippet: A derivative of pX330 (Addgene #42230) where the sgRNA cassette has been removed by NdeI digestion was used to express SpCas9 in mammalian cells (pX-Cas9). pX-Cas9 plasmids encoding the different mutants were obtained by site-directed mutagenesis of the WT pXCas9 plasmid using the ODNs reported in Table S2. sgRNAs were expressed from a pUC19 plasmid containing a U6-driven sgRNA expression cassette. .. Desired spacer sequences were cloned as annealed ODNs (Table S1) into a double BbsI site immediately upstream of the sgRNA scaffold according to previously published cloning strategies.35 To express SpCas9 in bacterial cells for recombinant protein production, a modified version of the Expressing:Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. All plasmids and primers used in the cloning experiments and oligonucleotides are summarized in Additional file 1: Table S2 and S3, respectively. Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. All plasmids and primers used in the cloning experiments and oligonucleotides are summarized in Additional file 1: Table S2 and S3, respectively. Clone Assay:Article Title: An optimized SpCas9 high-fidelity variant for direct protein delivery Article Snippet: A derivative of pX330 (Addgene #42230) where the sgRNA cassette has been removed by NdeI digestion was used to express SpCas9 in mammalian cells (pX-Cas9). pX-Cas9 plasmids encoding the different mutants were obtained by site-directed mutagenesis of the WT pXCas9 plasmid using the ODNs reported in Table S2. sgRNAs were expressed from a pUC19 plasmid containing a U6-driven sgRNA expression cassette. .. Desired spacer sequences were cloned as annealed ODNs (Table S1) into a double BbsI site immediately upstream of the sgRNA scaffold according to previously published cloning strategies.35 To express SpCas9 in bacterial cells for recombinant protein production, a modified version of the Modification:Article Title: An optimized SpCas9 high-fidelity variant for direct protein delivery Article Snippet: A derivative of pX330 (Addgene #42230) where the sgRNA cassette has been removed by NdeI digestion was used to express SpCas9 in mammalian cells (pX-Cas9). pX-Cas9 plasmids encoding the different mutants were obtained by site-directed mutagenesis of the WT pXCas9 plasmid using the ODNs reported in Table S2. sgRNAs were expressed from a pUC19 plasmid containing a U6-driven sgRNA expression cassette. .. Desired spacer sequences were cloned as annealed ODNs (Table S1) into a double BbsI site immediately upstream of the sgRNA scaffold according to previously published cloning strategies.35 To express SpCas9 in bacterial cells for recombinant protein production, a modified version of the |